911爆料网

Electrochemical biosensor of metabolites for in vitro investigations in biological samples

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Keyword

Electrochemical biosensor of metabolites for in vitro investigations in biological samples

Sukurta: 16 June 2025
Kryptis: Biochemija

The invention discloses an enzyme- and carbonaceous material (e.g. thermallyreduced graphene oxide (RGO))-based amperometric biosensor for direct evaluation of selected metabolites in biological media such as brain extract or serum. The biosensor comprises a working electrode (2), and a permeable membrane (1) covered with immobilized metabolite-selective enzyme with the carbonaceous material, being in contact with the electrode (2) surface, for bioelectrocatalytic oxidation of the metabolite, thus measuring its presence. The determination of a specific metabolite - L-glutamate - is based on registration at -0.1 V (vs. Ag/AgCl) of anodic current generated by the electrooxidation of ammonia released during the reaction catalyzed by L-glutamate oxidase, while L-glutamate is converting to alfa-ketoglutarate. The biosensor comprises a working graphite electrode (2) and adjustable semipermeable membrane (1) comprising L-glutamate oxidase (EC 1.4.3.11) immobilized into albumin and thermally reducated graphene oxide (RGO).

Inventors: Julija Razumien臈, Vidut臈 Gurevi膷ien臈, Ieva 艩akinyt臈-Urbikien臈, Marius Dagys, Dalius Ratautas.

Jurisdiction: EP.